Comparison of the effects of high dietary iron levels on bone microarchitecture responses in the mouse strains 129/Sv and C57BL/6J

Research output: Contribution to journalResearch articleContributedpeer-review


Iron is an essential nutrient for all living organisms. Both iron deficiency and excess can be harmful. Bone, a highly metabolic active organ, is particularly sensitive to fluctuations in iron levels. In this study, we investigated the effects of dietary iron overload on bone homeostasis with a specific focus on two frequently utilized mouse strains: 129/Sv and C57BL/6J. Our findings revealed that after 6 weeks on an iron-rich diet, 129/Sv mice exhibited a decrease in trabecular and cortical bone density in both vertebral and femoral bones, which was linked to reduced bone turnover. In contrast, there was no evidence of bone changes associated with iron overload in age-matched C57BL/6J mice. Interestingly, 129/Sv mice exposed to an iron-rich diet during their prenatal development were protected from iron-induced bone loss, suggesting the presence of potential adaptive mechanisms. Overall, our study underscores the critical role of genetic background in modulating the effects of iron overload on bone health. This should be considered when studying effects of iron on bone.


Original languageEnglish
Article number4887
JournalScientific reports
Issue number1
Publication statusPublished - Dec 2024

External IDs

PubMed 38418857
ORCID /0000-0002-8691-8423/work/157317837
ORCID /0000-0002-2061-8663/work/157319032


ASJC Scopus subject areas


  • 129/Sv, Bone loss, C57BL/6J, Iron-rich diet, Mouse strains, Bone and Bones, Mice, Inbred C57BL, Iron, Iron, Dietary, Mice, Inbred Strains, Pregnancy, Iron Overload/complications, Animals, Female, Mice

Library keywords