Comparative evaluation of microsatellite marker, AP-PCR and CGH studies in primary renal cell carcinoma.

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Christian Hoefling - , TUD Dresden University of Technology (Author)
  • Hannelore Schmidt - , TUD Dresden University of Technology (Author)
  • Matthias Meinhardt - , Department of Urology, Institute of Pathology (Author)
  • Andrea Lohse - , TUD Dresden University of Technology (Author)
  • Helge Taubert - , TUD Dresden University of Technology (Author)
  • Susanne Fuessel - , Department of Urology (Author)
  • Uta Schmidt - , TUD Dresden University of Technology (Author)
  • Katja Schuster - , TUD Dresden University of Technology (Author)
  • Gustavo Baretton - , Institute of Pathology (Author)
  • Manfred P. Wirth - , TUD Dresden University of Technology (Author)
  • Axel Meye - , TUD Dresden University of Technology (Author)

Abstract

Renal cancer consists of a heterogeneous tumor group which is characterized by complex cytogenetic and molecular genetic abnormalities. In this study, three different techniques were applied to screen renal cancers for genetic alterations. We studied 99 primary, sporadic renal cancers (96 renal cell carcinomas and three other renal cancers) by a comparative evaluation of different microsatellite markers, comparative genomic hybridization (CGH) and AP-PCR. The AP-PCR produces a genomic fingerprint after an AluEI DNA restriction digest of tumor DNA samples. Microsatellite alterations were investigated using nine microsatellite markers spanning well-known regions of FhiT and VHL (3p14.2, 3p26) but also of oncogenes and tumor suppressor genes like Myc-L1 and TP53Alu (1p32, 17p13.1). To receive a genomic fingerprint, AP-PCR was carried out for all patient samples. Performing AP-PCR, only one case out of 99 displayed genomic imbalance. Seven of 99 investigated primary renal cancers showed alterations in up to four microsatellite loci (TP53Alu, Myc-L1, D3S1300, D3S1560, D3S1317, D3S4260). Three markers (Bat25, Bat26, REN) did not reveal any aberrations within the tested tumor samples. Six cases with microsatellite alterations and four without were examined by CGH. Five samples yielded aberrations, four of them were positive for microsatellite alterations. Only one tumor sample displayed microsatellite alterations, shift patterns in AP-PCR and alterations analyzed by CGH. Our data suggest that genomic aberrations found by microsatellite analysis are also detectable by CGH with the restriction of a minimum of alterated DNA of >10 Mb. Based on this study of RCC and in contrast to other reports for solid tumors, we conclude that AP-PCR is far less informative in investigation of renal cancers.

Details

Original languageEnglish
Pages (from-to)835-842
Number of pages8
JournalInternational Journal of Molecular Medicine
Volume13
Issue number6
Publication statusPublished - Jun 2004
Peer-reviewedYes

External IDs

PubMed 15138621

Keywords

Sustainable Development Goals

ASJC Scopus subject areas