Cohesin SMC1β promotes closed chromatin and controls TERRA expression at spermatocyte telomeres

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Previous data showed that meiotic cohesin SMC1β protects spermatocyte telomeres from damage. The underlying reason, however, remained unknown as the expressions of telomerase and shelterin components were normal in Smc1β2/2 spermatocytes. Here. we report that SMC1β restricts expression of the long noncoding RNA TERRA (telomeric repeat containing RNA) in spermatocytes. In somatic cell lines increased TERRA was reported to cause telomere damage through altering telomere chromatin structure. In Smc1β2/2 spermatocytes, we observed strongly increased levels of TERRA which accumulate on damaged chromosomal ends, where enhanced R-loop formation was found. This suggested amore open chromatin configuration near telomeres in Smc1β2/2 spermatocytes, which was confirmed by ATAC-seq. Telomere-distal regions were not affected by the absence of SMC1β but RNA-seq revealed increased transcriptional activity in telomere-proximal regions. Thus, SMC1β promotes closed chromatin specifically near telomeres and limits TERRA expression in spermatocytes.

Details

Original languageEnglish
Article numbere202201798
Number of pages13
JournalLife science alliance
Volume6
Issue number7
Publication statusPublished - 1 Jul 2023
Peer-reviewedYes

External IDs

PubMed 37160312
WOS 000991258500001
ORCID /0000-0003-4306-930X/work/141545248
ORCID /0000-0002-3274-7163/work/142249711

Keywords

Keywords

  • Male, Chromatin/genetics, RNA, Long Noncoding/genetics, Spermatocytes, Telomere/genetics, Animals, Cell Cycle Proteins, Binding, Threats, R loops, Transcription, Antibody, Meiosis, Repeat-containing rna, Oocytes, Ctcf