Codon adaptation-based control of protein expression in C. elegans

Research output: Contribution to journal › Research article › Contributed › peer-review

Contributors

  • Stefanie Redemann - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Siegfried Schloissnig - (Author)
  • Susanne Ernst - (Author)
  • Andrey Pozniakowsky - (Author)
  • Swathi Ayloo - (Author)
  • Antony A Hyman - (Author)
  • Henrik Bringmann - , Chair of Cellular Circuits and Systems (Author)

Abstract

We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter.

Details

Original languageEnglish
Pages (from-to)250-252
Number of pages3
JournalNature methods
Volume8
Issue number3
Publication statusPublished - Mar 2011
Peer-reviewedYes

External IDs

Scopus 79952193455
ORCID /0000-0002-7689-8617/work/142237001

Keywords

Keywords

  • Animals, Caenorhabditis elegans/genetics, Caenorhabditis elegans Proteins/genetics, Cell Division/genetics, Codon/genetics, Gene Expression Regulation, Genetic Techniques, Protein Biosynthesis/genetics

Library keywords