Cloning and characterization of mammalian SMC1 and SMC3 genes and proteins, components of the DNA recombination complexes RC-1

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Members of the evolutionary conserved Structural Maintenance of Chromosomes (SMC) protein family are involved in chromosome condensation and gene dosage compensation with the SMC2 and SMC4 subtypes, and sister chromatid cohesion with the SMC1 and SMC3 subtypes. The bovine recombination protein complex RC-1, which catalyzes DNA transfer reactions, contains two heterodimeric SMC polypeptides, the genes of which have now been cloned, sequenced, and classified as bovine (b)SMC1 and bSMC3. Both proteins display all the characteristic features of the SMC family. FISH analysis localized the mouse SMC3 gene to chromosome 19D2-D3. Mono- and polyclonal antibodies specific for either subtype detected high levels of protein expression in lymphoid tissues, lung, testis and ovary. No change in levels of bSMC1 and bSMC3 proteins occurred after X-ray or UV-light irradiation of various cell lines or primary cells, and the amounts of individual proteins and the heterodimer are roughly constant throughout the cell cycle. Immunofluorescence of mouse cells detected the SMC1 protein in foci associated with the chromatin. These foci dissolve and the SMC protein dissociates from the chromatin during M phase.

Details

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalGene
Volume228
Issue number1-2
Publication statusPublished - 4 Mar 1999
Peer-reviewedYes

External IDs

Scopus 0033522135

Keywords

Keywords

  • Amino Acid Sequence, Animals, B-Lymphocytes/cytology, Cattle, Cell Cycle, Cell Cycle Proteins/genetics, Cell Nucleus/chemistry, Cells, Cultured, Chromosomal Proteins, Non-Histone, Chromosome Mapping, Cloning, Molecular, DNA Ligases/genetics, DNA, Complementary/chemistry, DNA-Directed DNA Polymerase/genetics, Endodeoxyribonucleases/genetics, Fluorescent Antibody Technique, Fungal Proteins/genetics, Genes/genetics, In Situ Hybridization, Fluorescence, Mammals/genetics, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Multienzyme Complexes/genetics, Nuclear Proteins/metabolism, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid