Circulating microRNAs predict recurrence and death following venous thromboembolism

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Vincent Ten Cate - , University Medical Center Mainz (Author)
  • Steffen Rapp - , University Medical Center Mainz (Author)
  • Andreas Schulz - , University Medical Center Mainz (Author)
  • Alejandro Pallares Robles - , University Medical Center Mainz (Author)
  • Kerstin Jurk - , University Medical Center Mainz (Author)
  • Thomas Koeck - , University Medical Center Mainz (Author)
  • Christine Espinola-Klein - , University Medical Center Mainz (Author)
  • Michael Halank - , Department of internal Medicine I, University Hospital Carl Gustav Carus Dresden (Author)
  • Hans-Jürgen Seyfarth - , Leipzig University (Author)
  • Manfred E Beutel - , University Medical Center Mainz (Author)
  • Alexander K Schuster - , University Medical Center Mainz (Author)
  • Federico Marini - , University Medical Center Mainz (Author)
  • Lukas Hobohm - , University Medical Center Mainz (Author)
  • Mareike Lankeit - , Charité – Universitätsmedizin Berlin (Author)
  • Karl J Lackner - , University Medical Center Mainz (Author)
  • Wolfram Ruf - , German Center for Cardiovascular Disease (DZHK) Partner site Mainz, Scripps Research Institute, University Medical Center Mainz (Author)
  • Thomas Münzel - , German Center for Cardiovascular Disease (DZHK) Partner site Mainz, University Medical Center Mainz (Author)
  • Miguel A Andrade-Navarro - , Johannes Gutenberg University Mainz (Author)
  • Jürgen H Prochaska - , University Medical Center Mainz (Author)
  • Stavros V Konstantinides - , Charité – Universitätsmedizin Berlin (Author)
  • Philipp S Wild - , University Medical Center Mainz (Author)

Abstract

BACKGROUND: Recurrent events frequently occur after venous thromboembolism (VTE) and remain difficult to predict based on established genetic, clinical, and proteomic contributors. The role of circulating microRNAs (miRNAs) has yet to be explored in detail.

OBJECTIVES: To identify circulating miRNAs predictive of recurrent VTE or death, and to interpret their mechanistic involvement.

METHODS: Data from 181 participants of a cohort study of acute VTE and 302 individuals with a history of VTE from a population-based cohort were investigated. Next-generation sequencing was performed on EDTA plasma samples to detect circulating miRNAs. The endpoint of interest was recurrent VTE or death. Penalized regression was applied to identify an outcome-relevant miRNA signature, and results were validated in the population-based cohort. The involvement of miRNAs in coregulatory networks was assessed using principal component analysis, and the associated clinical and molecular phenotypes were investigated. Mechanistic insights were obtained from target gene and pathway enrichment analyses.

RESULTS: A total of 1950 miRNAs were detected across cohorts after postprocessing. In the discovery cohort, 50 miRNAs were associated with recurrent VTE or death (cross-validated C-index, 0.65). A weighted miRNA score predicted outcome over an 8-year follow-up period (HRSD, 2.39; 95% CI, 1.98-2.88; P < .0001). The independent validation cohort validated 20 miRNAs (ORSD for score, 3.47; 95% CI, 2.37-5.07; P < .0001; cross-validated-area under the curve, 0.61). Principal component analysis revealed 5 miRNA networks with distinct relationships to clinical phenotype and outcome. Mapping of target genes indicated regulation via transcription factors and kinases involved in signaling pathways associated with fibrinolysis.

CONCLUSION: Circulating miRNAs predicted the risk of recurrence or death after VTE over several years, both in the acute and chronic phases.

Details

Original languageEnglish
Pages (from-to)2797-2810
Number of pages14
JournalJournal of Thrombosis and Haemostasis
Volume21
Issue number10
Publication statusPublished - Oct 2023
Peer-reviewedYes

External IDs

Scopus 85169022527

Keywords

Keywords

  • Humans, Circulating MicroRNA/genetics, Venous Thromboembolism/diagnosis, Cohort Studies, Proteomics, MicroRNAs/genetics