Cholesterol slows down the lateral mobility of an oxidized phospholipid in a supported lipid bilayer

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Birgit Plochberger - (Author)
  • Thomas Stockner - (Author)
  • Salvatore Chiantia - , Dresden University of Technology (Author)
  • Mario Brameshuber - (Author)
  • Julian Weghuber - (Author)
  • Albin Hermetter - (Author)
  • Petra Schwille - , Chair of Biophysics (Author)
  • Gerhard J. Schütz - (Author)

Abstract

We investigated the mobility and phase-partitioning of the fluorescent oxidized phospholipid analogue 1-palmitoyl-2-glutaroyl-sn-glycero-3-phospho-N- Alexa647-ethanolamine (PGPE-Alexa647) in supported lipid bilayers. Compared to the conventional phospholipid dihexadecanoylphosphoethanolamine (DHPE)-Bodipy we found consistently higher diffusion constants. The effect became dramatic when immobile obstacles were inserted into the bilayer, which essentially blocked the diffusion of DHPE-Bodipy but hardly influenced the movements of PGPE-Alexa647. In a supported lipid bilayer made of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the differences in probe mobility leveled off with increasing cholesterol content. Using coarse-grained molecular dynamics simulations, we could ascribe this effect to increased interactions between the oxidized phospholipid and the membrane matrix, concomitant with a translation in the headgroup position of the oxidized phospholipid: at zero cholesterol content, its headgroup is shifted to the outside of the DOPC headgroup region, whereas increasing cholesterol concentrations pulls the headgroup into the bilayer plane.

Details

Original languageEnglish
Pages (from-to)17322-17329
Number of pages8
JournalLangmuir
Volume26
Issue number22
Publication statusPublished - 16 Nov 2010
Peer-reviewedYes

External IDs

PubMed 20942393