Cholesterol slows down the lateral mobility of an oxidized phospholipid in a supported lipid bilayer

Research output: Contribution to journalResearch articleContributedpeer-review


  • Birgit Plochberger - (Author)
  • Thomas Stockner - (Author)
  • Salvatore Chiantia - , Dresden University of Technology (Author)
  • Mario Brameshuber - (Author)
  • Julian Weghuber - (Author)
  • Albin Hermetter - (Author)
  • Petra Schwille - , Chair of Biophysics (Author)
  • Gerhard J. Schütz - (Author)


We investigated the mobility and phase-partitioning of the fluorescent oxidized phospholipid analogue 1-palmitoyl-2-glutaroyl-sn-glycero-3-phospho-N- Alexa647-ethanolamine (PGPE-Alexa647) in supported lipid bilayers. Compared to the conventional phospholipid dihexadecanoylphosphoethanolamine (DHPE)-Bodipy we found consistently higher diffusion constants. The effect became dramatic when immobile obstacles were inserted into the bilayer, which essentially blocked the diffusion of DHPE-Bodipy but hardly influenced the movements of PGPE-Alexa647. In a supported lipid bilayer made of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the differences in probe mobility leveled off with increasing cholesterol content. Using coarse-grained molecular dynamics simulations, we could ascribe this effect to increased interactions between the oxidized phospholipid and the membrane matrix, concomitant with a translation in the headgroup position of the oxidized phospholipid: at zero cholesterol content, its headgroup is shifted to the outside of the DOPC headgroup region, whereas increasing cholesterol concentrations pulls the headgroup into the bilayer plane.


Original languageEnglish
Pages (from-to)17322-17329
Number of pages8
Issue number22
Publication statusPublished - 16 Nov 2010

External IDs

PubMed 20942393