Chlorogenic Acid Protects DNA against Double-strand Breaks: Evidence from Single Molecule Observation

Research output: Contribution to journal › Review article › Contributed › peer-review

Contributors

  • Haruto Ogawa - , Doshisha University (Author)
  • Takashi Nishio - , Chair of Theoretical Physics of Living Matter (PoL), Doshisha University (Author)
  • Yuko Yoshikawa - , Doshisha University (Author)
  • Koichiro Sadakane - , Doshisha University (Author)
  • Kenichi Yoshikawa - , Doshisha University, Kyoto University (Author)

Abstract

A natural polyphenol chlorogenic acid (CGA) is regarded as an antioxidant by preventing oxidative damage. Among the oxidative damages, DNA double-strand breaks (DSBs) are considered the most severe. To study the protective effect of CGA against DSBs, we measured the time-dependent increase of DSBs caused by photo-irradiation through a single molecule observation of DNA (166 kbp) using fluorescence microscopy. The results have been analyzed in relation to the two-step mechanism with single-strand breaks.

Details

Original languageEnglish
Pages (from-to)524-527
Number of pages4
JournalChemistry letters
Volume52
Issue number6
Publication statusPublished - Jun 2023
Peer-reviewedYes

External IDs

ORCID /0000-0001-8761-1776/work/161887582

Keywords

ASJC Scopus subject areas

Keywords

  • Antioxidant chlorogenic acid, DNA double-strand breaks, Single-DNA observation