We investigate the charge-order transition at zero temperature in a two-leg Hubbard ladder with additional nearest-neighbor Coulomb repulsion V using the density matrix renormalization group technique. We consider electron densities between quarter and half filling. For quarter filling and U=8t, we find evidence for a continuous phase transition between a homogeneous state at small V and a broken-symmetry state with “checkerboard” [wave vector Q=(π,π)] charge order at large V. This transition to a checkerboard charge-ordered state remains present at all larger fillings, but becomes discontinuous at sufficiently large filling. We discuss the influence of U/t on the transition and estimate the position of the tricritical points.