Cellular, intracellular, and developmental expression patterns of murine SWAP-70

Research output: Contribution to journalResearch articleContributedpeer-review


  • T Borggrefe - , Basel Institute for Immunology (Author)
  • L Masat - (Author)
  • M Wabl - (Author)
  • B Riwar - (Author)
  • G Cattoretti - (Author)
  • R Jessberger - , Institute of Physiological Chemistry (Author)


SWAP-70 is part of a protein complex that catalyzes cell-free DNA recombination between immunoglobulin heavy chain gene switch region substrates. This report studies the expression pattern of SWAP-70 in mouse tissues, sorted cells, and cultured primary cells. SWAP-70 RNA is strongly increased upon switch-induction of spleen cells, and very weakly expressed in thymus and bone marrow. SWAP-70 protein is specifically expressed in B cells, and levels increase rapidly after stimulation. Tissue staining shows strong expression in germinal center B cells, while macrophages and T lymphocytes do not stain. SWAP-70 is not detected in early B cells in the bone marrow. Its expression during mouse ontogeny after birth correlates with the appearance of non-IgM isotypes. While SWAP-70 localizes to the cell nucleus in activated B cells, it is not tightly associated with the chromatin and is found in the cytoplasm as well. SWAP-70 expression is not increased by gamma or UV irradiation of spleen cells, nor does it depend on p53. These characteristics are consistent with the putative role of SWAP-70 in immunoglobulin class switching.


Original languageEnglish
Pages (from-to)1812-22
Number of pages11
JournalEuropean Journal of Immunology
Issue number6
Publication statusPublished - Jun 1999

External IDs

Scopus 0033040306



  • Animals, B-Lymphocytes/cytology, Cell Differentiation, Cells, Cultured, DNA-Binding Proteins/biosynthesis, Female, Gene Expression Regulation, Developmental/radiation effects, Guanine Nucleotide Exchange Factors, Male, Mice, Mice, Inbred C57BL, Minor Histocompatibility Antigens, Nuclear Proteins/biosynthesis, Pregnancy, RNA, Messenger/genetics, Recombination, Genetic, Subcellular Fractions/metabolism, Tissue Distribution