Cellular, intracellular, and developmental expression patterns of murine SWAP-70
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
SWAP-70 is part of a protein complex that catalyzes cell-free DNA recombination between immunoglobulin heavy chain gene switch region substrates. This report studies the expression pattern of SWAP-70 in mouse tissues, sorted cells, and cultured primary cells. SWAP-70 RNA is strongly increased upon switch-induction of spleen cells, and very weakly expressed in thymus and bone marrow. SWAP-70 protein is specifically expressed in B cells, and levels increase rapidly after stimulation. Tissue staining shows strong expression in germinal center B cells, while macrophages and T lymphocytes do not stain. SWAP-70 is not detected in early B cells in the bone marrow. Its expression during mouse ontogeny after birth correlates with the appearance of non-IgM isotypes. While SWAP-70 localizes to the cell nucleus in activated B cells, it is not tightly associated with the chromatin and is found in the cytoplasm as well. SWAP-70 expression is not increased by gamma or UV irradiation of spleen cells, nor does it depend on p53. These characteristics are consistent with the putative role of SWAP-70 in immunoglobulin class switching.
Details
Original language | English |
---|---|
Pages (from-to) | 1812-22 |
Number of pages | 11 |
Journal | European Journal of Immunology |
Volume | 29 |
Issue number | 6 |
Publication status | Published - Jun 1999 |
Peer-reviewed | Yes |
External IDs
Scopus | 0033040306 |
---|
Keywords
Keywords
- Animals, B-Lymphocytes/cytology, Cell Differentiation, Cells, Cultured, DNA-Binding Proteins/biosynthesis, Female, Gene Expression Regulation, Developmental/radiation effects, Guanine Nucleotide Exchange Factors, Male, Mice, Mice, Inbred C57BL, Minor Histocompatibility Antigens, Nuclear Proteins/biosynthesis, Pregnancy, RNA, Messenger/genetics, Recombination, Genetic, Subcellular Fractions/metabolism, Tissue Distribution