BMP-4 and Noggin signaling modulate dorsal fin and somite development in the axolotl trunk

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

BMP-4, a member of the TGF-beta superfamily of growth factors, is involved in various developmental processes. We investigated the effects of BMP-4 and its antagonist Noggin on axolotl trunk development. Implantation of BMP-4-coated microbeads caused inhibition of muscle and dorsal fin formation in the vicinity of the microbeads. At some distance, myotomes developed with reduced height but increased width, which was accompanied by increased cell proliferation. These effects could be modulated by co-implanting Noggin-coated beads. Immunostaining of Pax7 further revealed that although the dermomyotome was absent in the vicinity of BMP-4-coated beads, at some distance from them, it was thicker than in controls, indicating that moderate amounts of BMP-4 stimulate this layer of undifferentiated cells. In contrast, Noggin generally inhibited the dermomyotome, possibly indicating premature differentiation of dermomyotome cells. We conclude that BMP-4 and Noggin are involved in the regulation of cell proliferation and differentiation during somite development.

Details

Original languageEnglish
Pages (from-to)2464-2474
Number of pages11
JournalDevelopmental Dynamics
Volume236
Issue number9
Publication statusPublished - Sept 2007
Peer-reviewedYes

External IDs

Scopus 34548851981
researchoutputwizard legacy.publication#18681
researchoutputwizard legacy.publication#18388
PubMed 17654602
ORCID /0000-0001-5624-1717/work/142238989

Keywords

Keywords

  • Ambystoma/embryology, Animals, Bone Morphogenetic Protein 4, Bone Morphogenetic Proteins/physiology, Carrier Proteins/physiology, Cell Differentiation, Cell Proliferation, Dermis/embryology, Developmental Biology/methods, Extremities/physiology, Fishes, Gene Expression Regulation, Developmental, Models, Biological, Muscle Development, Somites/metabolism, Xenopus, Xenopus Proteins