The aim of this study is to investigate the relationship between bioclimatic comfort and land use in İzmir by using geographical information systems and remote sensing technologies. In this context, it is aimedto evaluate the relationship between the bioclimatic conditions of the years 1990, 2000, 2006, 2012and 2018 with the use of land in the same years in the province of İzmir in seasonal and annual periods.Physiological Equivalent Temperature (PET) index, which takes into consideration the physiologicalcharacteristics of human, was used when determining bioclimatic comfort zones. Inverse DistanceWeighting (IDW) method was preferred in the calculation of the spatial distribution of the obtainedvalues at sea level. Height-dependent PET values were obtained and bioclimatic comfort maps weregenerated. Land use maps were created by using the bioclimatic comfort maps produced by referenceto the years with CORINE land cover data. As a result of the study, it was observed that the vast majority of the comfort zones consisted of agricultural areas and then natural and semi-natural areas, forestareas and settlements, respectively. It has been determined that the temperature difference in forestareas is less than the land areas, but it is more than urban heat islands and the city's cooling effectcannot be used due to the high humidity in the city and the distorted urbanization observed in the citycenter.