Biosynthesis of ZnO, Bi2O3 and ZnO−Bi2O3 bimetallic nanoparticles and their cytotoxic and antibacterial effects

Research output: Contribution to journalResearch articleContributedpeer-review


  • Mina Sarani - , Iran Polymer and Petrochemical Institute (Author)
  • Majid Darroudi - , Mashhad University of Medical Sciences, Neyshabur University of Medical Sciences (Author)
  • Mahin Naderifar - , Zabol University of Medical Sciences (Author)
  • Majid Reza Akbarizadeh - , Zabol University of Medical Sciences (Author)
  • Marcos A.L. Nobre - , Universidade Estadual Paulista Júlio de Mesquita Filho (Author)
  • Benjamin Kruppke - , Chair of Biomaterials (Author)
  • Hossein Ali Khonakdar - , Chair of Biomaterials, Iran Polymer and Petrochemical Institute (Author)
  • Mehdi Erfani Jazi - , Bowling Green State University (Author)


This work introduces an easy method for producing Bi2O3, ZnO, ZnO-Bi2O3 nanoparticles (NPs) by Biebersteinia Multifida extract. Our products have been characterized through the outcomes which recorded with using powder X-ray diffractometry (PXRD), Raman, energy dispersive X-ray (EDX), field emission-scanning electron microscopy (FE-SEM), and Fourier-transform infrared (FT-IR) techniques. The finding of SEM presented porous structure and spherical morphology for Bi2O3 and ZnO NPs, respectively. While FE-SEM image of bimetallic nanoparticles showed both porous and spherical morphologies for them; so that spherical particles of ZnO have sat on the porous structure of Bi2O3 NPs. According to the PXRD results, the crystallite sizes of Bi2O3, ZnO and ZnO−Bi2O3 NPs have been obtained 57.69, 21.93, and 43.42 nm, respectively. Antibacterial performance of NPs has been studied on Staphylococcus epidermidis and Pseudomonas aeruginosa bacteria, to distinguish the minimum microbial inhibitory concentration (MIC). Antimicrobial outcomes have showed a better effect for ZnO-Bi2O3 NPs. Besides, wondering about the cytotoxic action against cancer cell lines, the MTT results have verified the intense cytotoxic function versus breast cancer cells (MCF-7). According to these observations, obtained products can prosper medical and biological applications.


Original languageEnglish
Article numbere202300176
Number of pages11
Issue number4
Early online dateJan 2024
Publication statusPublished - Apr 2024

External IDs

WOS 001144047800001
PubMed 38230849
Mendeley 80492d35-cbd1-3b60-8f30-606ed8af3c4e


Sustainable Development Goals

ASJC Scopus subject areas


  • Biebersteinia Multifida, MCF-7, MTT, S. epidermidis, ZnO−Bi2O3 NP, ZnO−BiO NP, Anti-Infective Agents/pharmacology, Antineoplastic Agents/pharmacology, Zinc Oxide/pharmacology, Nanoparticles/chemistry, Spectroscopy, Fourier Transform Infrared, Anti-Bacterial Agents/pharmacology