Bioinspired calcium phosphate mineralization on net-shape-nonwoven chitosan scaffolds stimulates human bone marrow stromal cell differentiation

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Chitosan fibers were processed using the Net-Shape-Nonwoven (NSN) technique in order to create porous scaffolds which were functionalized in two bioinspired ways: collagen type I coating and unique mineralization with organically modified hydroxyapatite (ormoHAP). While collagen is common to enhance cell attachment on surfaces, the electric-field assisted migration and deposition of ormoHAP on the surface of the NSN-scaffolds is a novel technique which enables sub-micrometer sized mineralization while maintaining the original pore structure. Microscopy revealed fast attachment and morphological adaptation of the cells on both, the pure and the functionalized NSN-scaffolds. Remarkably, the cell number of osteogenically induced hBMSC on ormoHAP-modified NSN-scaffolds increased 3.5–5 fold compared to pure NSN-scaffolds. Osteogenic differentiation of hBMSC/osteoblasts was highest on collagen-functionalized NSN-scaffolds. RT-PCR studies revealed gene expression of ALP, BSP II, and osteocalcin to be high for all NSN-scaffolds. Overall, the NSN-scaffold functionalization with collagen and ormoHAP improved attachment, proliferation, and differentiation of hBMSC and therefore revealed the remarkable potential of their application for the tissue engineering of bone.

Details

Original languageEnglish
Article number045017
JournalBiomedical Materials (Bristol)
Volume14
Issue number4
Publication statusPublished - 2019
Peer-reviewedYes

External IDs

Scopus 85068489456
researchoutputwizard legacy.publication#85207
ORCID /0000-0002-7440-9277/work/142239078

Keywords

Keywords

  • net-shape-nonwoven chitosan scaffolds