Bioinks for Space Missions: The Influence of Long‐Term Storage of Alginate‐Methylcellulose‐Based Bioinks on Printability as well as Cell Viability and Function

Research output: Contribution to journalResearch articleContributedpeer-review


Bioprinting is considered a key technology for future space missions and is currently being established on the International Space Station (ISS). With the aim to perform bioink production as a critical and resource-consuming preparatory step already on Earth and transport a bioink cartridge “ready to use” to the ISS, the storability of bioinks is investigated. Hydrogel blends based on alginate and methylcellulose are laden with either green microalgae of the species Chlorella vulgaris or with different human cell lines including immortilized human mesenchymal stem cells, SaOS-2 and HepG2, as well as with primary human dental pulp stem cells. The bioinks are filled into printing cartridges and stored at 4°C for up to four weeks. Printability of the bioinks is maintained after storage. Viability and function of the cells embedded in constructs bioprinted from the stored bioinks are investigated during subsequent cultivation: The microalgae survive the storage period very well and show no loss of growth and functionality, however a significant decrease is visible for human cells, varying between the different cell types. The study demonstrates that storage of bioinks is in principle possible and is a promising starting point for future research, making complex printing processes more effective and reproducible.


Original languageEnglish
Article number2300436
JournalAdvanced healthcare materials
Issue number23
Publication statusPublished - 12 May 2023

External IDs

Scopus 85159060272
WOS 000986349400001
Mendeley 65381eb5-2aaa-3ffc-a91e-f014abfff6f3


Research priority areas of TU Dresden


  • Bioink storability, Bioprinting, Cold storage, Launch, Microalgae, cold storage, microalgae, bioink storability, launch, bioprinting, cold storages