A chemical method to obtain purely organic materials with room temperature phosphorescence and tuned polarized emission is developed. The chirality of the asymmetry center can be precisely controlled bottom-up exhibiting either a reserved or inverted configuration, without enantiomer separation procedures. Developed luminescent materials with varied chiral configurations show polarized photoluminescence and room temperature phosphorescence up to seconds, which can be used for time- and polarization dependent microscopy applications, with an integrated linear polarization anisotropy factor as large as 0.44.