Association of SWAP-70 with the B cell antigen receptor complex

Research output: Contribution to journal › Research article › Contributed › peer-review

Contributors

  • L Masat - , University of California at Irvine (Author)
  • J Caldwell - (Author)
  • R Armstrong - (Author)
  • H Khoshnevisan - (Author)
  • R Jessberger - , Institute of Physiological Chemistry (Author)
  • B Herndier - (Author)
  • M Wabl - (Author)
  • D Ferrick - (Author)

Abstract

SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. We report here the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. We suggest that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation.

Details

Original languageEnglish
Pages (from-to)2180-4
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America : PNAS
Volume97
Issue number5
Publication statusPublished - 29 Feb 2000
Peer-reviewedYes

External IDs

PubMedCentral PMC15774
Scopus 0034008135

Keywords

Keywords

  • Amino Acid Sequence, Animals, Biological Transport, CD40 Antigens/metabolism, DNA-Binding Proteins/genetics, Guanine Nucleotide Exchange Factors, Humans, Immunoglobulin G/immunology, Immunoglobulin M/immunology, Minor Histocompatibility Antigens, Molecular Sequence Data, Mutagenesis, Nuclear Proteins/genetics, Rabbits, Receptors, Antigen, B-Cell/metabolism, Tissue Distribution, Tumor Cells, Cultured