Arrested coalescence of multicellular aggregates

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • David Oriola - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Miquel Marin-Riera - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Kerim Anlaş - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Nicola Gritti - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Marina Sanaki-Matsumiya - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Germaine Aalderink - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Miki Ebisuya - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • James Sharpe - , ICREA (Author)
  • Vikas Trivedi - , European Molecular Biology Laboratory (EMBL) Heidelberg (Author)

Abstract

Multicellular aggregates are known to exhibit liquid-like properties. The fusion process of two cell aggregates is commonly studied as the coalescence of two viscous drops. However, tissues are complex materials and can exhibit viscoelastic behaviour. It is known that elastic effects can prevent the complete fusion of two drops, a phenomenon known as arrested coalescence. Here we study this phenomenon in stem cell aggregates and provide a theoretical framework which agrees with the experiments. In addition, agent-based simulations show that active cell fluctuations can control a solid-to-fluid phase transition, revealing that arrested coalescence can be found in the vicinity of an unjamming transition. By analysing the dynamics of the fusion process and combining it with nanoindentation measurements, we obtain the effective viscosity, shear modulus and surface tension of the aggregates. More generally, our work provides a simple, fast and inexpensive method to characterize the mechanical properties of viscoelastic materials.

Details

Original languageEnglish
Pages (from-to)3771-3780
Number of pages10
JournalSoft matter
Volume18
Issue number19
Publication statusPublished - 18 May 2022
Peer-reviewedYes
Externally publishedYes

External IDs

Scopus 85130767194

Keywords

Keywords

  • Surface Tension, Viscosity