Argon protects hypoxia-, cisplatin- and gentamycin-exposed hair cells in the newborn rat's organ of Corti

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Yury M. Yarin - , Charité – Universitätsmedizin Berlin, University Hospital Carl Gustav Carus Dresden, Department of Otorhinolaryngology, Head and Neck Surgery (Author)
  • Nyamaa Amarjargal - , Charité – Universitätsmedizin Berlin, Mongolian National University of Medical Sciences (Author)
  • Julia Fuchs - , Charité – Universitätsmedizin Berlin (Author)
  • Heidemarie Haupt - , Charité – Universitätsmedizin Berlin (Author)
  • Birgit Mazurek - , Charité – Universitätsmedizin Berlin (Author)
  • Svetlana V. Morozova - , Sechenov First Moscow State Medical University (Author)
  • Johann Gross - , Charité – Universitätsmedizin Berlin (Author)

Abstract

During the last few years, an important protective effect of the noble gas xenon against neuronal hypoxic damage was observed. However, argon (Ar), a gas from the same chemical group, but less expensive and without anesthetic effect at normobaric pressure, has not been studied in terms of possible biological effects on cell protection. Ar was tested for its ability to protect organotypic cultures of the organ of Corti from 3-5 day old rats against hypoxia, cisplatin, and gentamycin toxicity. Cultures were exposed to nitrogen hypoxia (5% CO2, 95% N2), Ar hypoxia (5% CO2, 95% Ar) or normoxia for 30 h. Ar protected the hair cells from hypoxia-induced damage by about 25%. Ar-oxygen (O2) mixtures (21% O2, 5% CO 2, 74% Ar) had no effect on the hair cell survival. Cisplatin (7.5-25 μM) and gentamycin (5-40 μM) exposed in medium under air damaged the hair cells in a dose-dependent manner. The exposure of cisplatin- and gentamycin-treated cultures to the Ar-O2 atmosphere significantly reduced the hair cell damage by up to 25%. This protective effect of Ar might provide a new protective approach against ototoxic processes.

Details

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalHearing research
Volume201
Issue number1-2
Publication statusPublished - Mar 2005
Peer-reviewedYes

External IDs

Scopus 13844272413

Keywords

Keywords

  • Argon, Cisplatin, Gentamycin, Hair cells, Hypoxia, Ototoxicity