Anti-skeletal muscle atrophy effect of Oenothera odorata root extract via reactive oxygen species-dependent signaling pathways in cellular and mouse model
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Skeletal muscle atrophy can be defined as a decrease of muscle volume caused by injury or lack of use. This condition is associated with reactive oxygen species (ROS), resulting in various muscular disorders. We acquired 2D and 3D images using micro-computed tomography in gastrocnemius and soleus muscles of sciatic-denervated mice. We confirmed that sciatic denervation-small animal model reduced muscle volume. However, the intraperitoneal injection of Oenothera odorata root extract (EVP) delayed muscle atrophy compared to a control group. We also investigated the mechanism of muscle atrophy's relationship with ROS. EVP suppressed expression of SOD1, and increased expression of HSP70, in both H2O2-treated C2C12 myoblasts and sciatic-denervated mice. Moreover, EVP regulated apoptotic signals, including caspase- 3, Bax, Bcl-2, and ceramide. These results indicate that EVP has a positive effect on reducing the effect of ROS on muscle atrophy.
Details
Original language | English |
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Pages (from-to) | 80-88 |
Number of pages | 9 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 80 |
Issue number | 1 |
Publication status | Published - 2016 |
Peer-reviewed | Yes |
External IDs
PubMed | 26613402 |
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ORCID | /0000-0001-9075-5121/work/160951479 |
Keywords
ASJC Scopus subject areas
Keywords
- Heat shock protein 71, Muscle atrophy, Oenothera odorata, Reactive oxygen species, Superoxide dismutase