Analysis of Electric Field Stimulation in Blue Light Stressed 661W Cells

Research output: Contribution to journalResearch articleContributedpeer-review



Though electrical stimulation is used as a therapeutic approach to treat retinal and spinalinjuries, many protective mechanisms at cellular level have not been elucidated. We performeda detailed analysis of cellular events in blue light (Li) stressed 661W cells, which were subjectedto direct current electric field (EF) stimulation. Our findings revealed that EF stimulation inducedprotective effects in 661W cells from Li-induced stress by multiple defense mechanisms, such asincrease in mitochondrial activity, gain in mitochondrial potential, increase in superoxide levels, andthe activation of unfolded protein response (UPR) pathways, all leading to an enhanced cell viabilityand decreased DNA damage. Here, our genetic screen results revealed the UPR pathway to be apromising target to ameliorate Li-induced stress by EF stimulation. Thus, our study is important fora knowledgeable transfer of EF stimulation into clinical application.


Original languageEnglish
Article number3433
Number of pages19
JournalInternational journal of molecular sciences
Issue number4
Publication statusPublished - 8 Feb 2023

External IDs

unpaywall 10.3390/ijms24043433
Scopus 85149050679
PubMed 36834840


DFG Classification of Subject Areas according to Review Boards

Subject groups, research areas, subject areas according to Destatis


  • blue light, electrical stimulation, membrane potential, photoreceptors, unfolded protein response, Cell Line, Mitochondria, Electric Stimulation, Light, Retina, Unfolded Protein Response