An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: A note of caution, Austria, Germany, Ireland, 2020

Research output: Contribution to journal › Research article › Contributed › peer-review

Contributors

  • Stefan Monecke - , Leibniz Institute of Photonic Technology, TUD Dresden University of Technology, InfectoGnostics Research Campus Jena, Reg. Assoc. (Author)
  • Elisabeth König - , Medical University of Graz (Author)
  • Megan R. Earls - , Dublin Dental University Hospital (Author)
  • Eva Leitner - , Medical University of Graz (Author)
  • Elke Müller - , Leibniz Institute of Photonic Technology, InfectoGnostics Research Campus Jena, Reg. Assoc. (Author)
  • Gabriel E. Wagner - , Medical University of Graz (Author)
  • David M. Poitz - , Institute of Clinical Chemistry and Laboratory Medicine, TUD Dresden University of Technology (Author)
  • Lutz Jatzwauk - , TUD Dresden University of Technology (Author)
  • Teodora Vremera - , Grigore T. Popa University of Medicine and Pharmacy (Author)
  • Olivia S. Dorneanu - , Grigore T. Popa University of Medicine and Pharmacy (Author)
  • Alexandra Simbeck - , University of Regensburg (Author)
  • Andreas Ambrosch - , Hospital of the Brothers of Mercy Regensburg (Author)
  • Ines Zollner-Schwetz - , Medical University of Graz (Author)
  • Robert Krause - , Medical University of Graz (Author)
  • Werner Ruppitsch - , Austrian Agency for Health and Food Safety GmbH (Author)
  • Wulf Schneider-Brachert - , University of Regensburg (Author)
  • David C. Coleman - , Dublin Dental University Hospital (Author)
  • Ivo Steinmetz - , Medical University of Graz (Author)
  • Ralf Ehricht - , Leibniz Institute of Photonic Technology, InfectoGnostics Research Campus Jena, Reg. Assoc., Friedrich Schiller University Jena (Author)

Abstract

We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites.

Details

Original languageEnglish
Article number2000929
JournalEurosurveillance
Volume25
Issue number24
Publication statusPublished - Jun 2020
Peer-reviewedYes

External IDs

PubMed 32613938
ORCID /0000-0001-7803-1972/work/142235089

Keywords

Sustainable Development Goals

ASJC Scopus subject areas