An approach to prepare membrane proteins for single-molecule imaging

Research output: Contribution to journal › Research article › Contributed › peer-review

Contributors

  • David A. Cisneros - , TUD Dresden University of Technology (Author)
  • Daniel J. Muller - , Chair of Cellular Machines (Author)
  • Sofian M. Daud - (Author)
  • Jeremy H. Lakey - (Author)

Abstract

(Figure Presented) Observed from above: A cysteine mutation in a membrane protein enables it to be fixed in a defined orientation on flat gold surfaces (see picture, left). Subsequent coassembly with thiolipids produces clearly defined, immobilized proteins. Analysis by atomic force microscopy provides topographs of similar quality to those revealed from 2D crystals.

Details

Original languageEnglish
Pages (from-to)3252-3256
Number of pages5
JournalAngewandte Chemie - International Edition
Volume45
Issue number20
Publication statusPublished - 12 May 2006
Peer-reviewedYes

External IDs

PubMed 16634099

Keywords

ASJC Scopus subject areas

Keywords

  • Lipids, Membrane proteins, Porin OmpF, Protein structures, Scanning force microscopy