Agreement of steroid profiles in Athlete Biological Passport residues and corresponding serum samples

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Simon König - , Institute of Doping Analysis and Sports Biochemistry Dresden (First author)
  • Sebastian Rzeppa - , Institute of Doping Analysis and Sports Biochemistry Dresden (Author)
  • Detlef Thieme - , Institute of Doping Analysis and Sports Biochemistry Dresden (Author)
  • Annekathrin M Keiler - , Environmental Monitoring and Endocrinology (Research Group), Faculty of Biology, Institute of Doping Analysis and Sports Biochemistry Dresden (Last author)

Abstract

The steroid module of the Athlete Biological Passport (ABP) is based on the analysis of six endogenous steroids in urine samples and a Bayesian statistical approach. However, the urinary steroid concentrations may be affected by confounders like microbial degradation, possible co-administration of diuretics as masking agents, insufficient conjugate hydrolysis or UGT2B17 gene polymorphisms affecting glucuronidation. Therefore, it can be helpful to use other matrices (ABP blood and serum samples) to quantify steroids and thereby support noticeable deviations in the Athlete Biological Passport, for example, abnormally increased urinary testosterone/epitestosterone (T/E) ratios. Aim of the study was to investigate the feasibility to re-use plasma obtained from athlete ABP blood samples for measuring a steroid profile. Therefore, testosterone, androstenedione, cortisol and cortisone were quantified in 36 intra-individual matching ABP blood and serum samples. The steroid levels measured in both matrices showed a high agreement indicating a good stability uninfluenced by storage temperature and duration. Our results pointed out the possibility to expand the athlete ABP blood analysis for steroid profiling.

Details

Original languageEnglish
JournalDrug testing and analysis
Early online date6 Sep 2022
Publication statusPublished - 8 Sep 2022
Peer-reviewedYes

External IDs

unpaywall 10.1002/dta.3365
Scopus 85137516403
WOS 000851491900001
Mendeley e048fa4e-bbea-39c8-bee2-76f2517b2360

Keywords

Keywords

  • athlete biological passport, blood, stability, steroids