Recently, there has been remarkable progress in the development of smart textiles, especially knitted strain sensors, to achieve reliable sensor signals. Stable and reliable electro-mechanical properties of sensors are essential for using textile-based sensors in medical applications. However, the challenges associated with significant hysteresis and low gauge factor (GF) values remain for using strain sensors for motion capture. To evaluate these issues, a comprehensive investigation of the cyclic electro-mechanical properties of weft-knitted strain sensors was conducted in the present study to develop a drift-free elastic strain sensor with a robust sensor signal for motion capture for medical devices. Several variables are considered in the study, including the variation of the basic knit pattern, the incorporation of the electrically conductive yarn, and the size of the strain sensor. The effectiveness and feasibility of the developed knitted strain sensors are demonstrated through an experimental evaluation, by determining the gauge factor, its nonlinearity, hysteresis, and drift. The developed knitted piezoresistive strain sensors have a GF of 2.4, a calculated drift of 50%, 12.5% hysteresis, and 0.3% nonlinearity in parts.