Adipose cells and tissues soften with lipid accumulation while in diabetes adipose tissue stiffens

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Adipose tissue expansion involves both differentiation of new precursors and size increase of mature adipocytes. While the two processes are well balanced in healthy tissues, obesity and diabetes type II are associated with abnormally enlarged adipocytes and excess lipid accumulation. Previous studies suggested a link between cell stiffness, volume and stem cell differentiation, although in the context of preadipocytes, there have been contradictory results regarding stiffness changes with differentiation. Thus, we set out to quantitatively monitor adipocyte shape and size changes with differentiation and lipid accumulation. We quantified by optical diffraction tomography that differentiating preadipocytes increased their volumes drastically. Atomic force microscopy (AFM)-indentation and -microrheology revealed that during the early phase of differentiation, human preadipocytes became more compliant and more fluid-like, concomitant with ROCK-mediated F-actin remodelling. Adipocytes that had accumulated large lipid droplets were more compliant, and further promoting lipid accumulation led to an even more compliant phenotype. In line with that, high fat diet-induced obesity was associated with more compliant adipose tissue compared to lean animals, both for drosophila fat bodies and murine gonadal adipose tissue. In contrast, adipose tissue of diabetic mice became significantly stiffer as shown not only by AFM but also magnetic resonance elastography. Altogether, we dissect relative contributions of the cytoskeleton and lipid droplets to cell and tissue mechanical changes across different functional states, such as differentiation, nutritional state and disease. Our work therefore sets the basis for future explorations on how tissue mechanical changes influence the behaviour of mechanosensitive tissue-resident cells in metabolic disorders.


Original languageEnglish
Article number10325
Number of pages17
JournalScientific reports
Issue number1
Publication statusPublished - 20 Jun 2022

External IDs

PubMed 35725987
WOS 000838090200069
unpaywall 10.1038/s41598-022-13324-9


Research priority areas of TU Dresden

DFG Classification of Subject Areas according to Review Boards

Sustainable Development Goals

ASJC Scopus subject areas


  • Adipocytes/metabolism, Adipose Tissue/metabolism, Animals, Cell Differentiation, Diabetes Mellitus, Experimental/metabolism, Lipids, Mice, Obesity/metabolism, STEM-CELLS, ADIPOGENESIS, MICROSCOPY, MECHANICAL-PROPERTIES, STRESS FIBERS, STIFFNESS, INSULIN-RESISTANCE, INFLAMMATION, RHO, ADIPOCYTE DIFFERENTIATION

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