A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
The process of cardiac morphogenesis in humans is incompletely understood. Its full characterization requires a deep exploration of the organ-wide orchestration of gene expression with a single-cell spatial resolution. Here, we present a molecular approach that reveals the comprehensive transcriptional landscape of cell types populating the embryonic heart at three developmental stages and that maps cell-type-specific gene expression to specific anatomical domains. Spatial transcriptomics identified unique gene profiles that correspond to distinct anatomical regions in each developmental stage. Human embryonic cardiac cell types identified by single-cell RNA sequencing confirmed and enriched the spatial annotation of embryonic cardiac gene expression. In situ sequencing was then used to refine these results and create a spatial subcellular map for the three developmental phases. Finally, we generated a publicly available web resource of the human developing heart to facilitate future studies on human cardiogenesis.
Details
Original language | English |
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Pages (from-to) | 1647-1660.e19 |
Journal | Cell |
Volume | 179 |
Issue number | 7 |
Publication status | Published - 12 Dec 2019 |
Peer-reviewed | Yes |
External IDs
Scopus | 85076165520 |
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ORCID | /0000-0003-1065-4107/work/141543984 |
Keywords
Keywords
- Female, Gene Expression Regulation, Developmental, Heart/embryology, Humans, Male, Morphogenesis, Myocytes, Cardiac/cytology, RNA-Seq, Single-Cell Analysis, Transcriptome