A Self‐Assembled Matrix System for Cell‐Bioengineering Applications in Different Dimensions, Scales, and Geometries

Research output: Contribution to journalResearch articleContributedpeer-review



Stem cell bioengineering and therapy require different model systems and materials in different stages of development. If a chemically defined biomatrix system can fulfill most tasks, it can minimize the discrepancy among various setups. By screening biomaterials synthesized through a coacervation-mediated self-assembling mechanism, a biomatrix system optimal for 2D human mesenchymal stromal cell (hMSC) culture and osteogenesis is identified. Its utility for hMSC bioengineering is further demonstrated in coating porous bioactive glass scaffolds and nanoparticle synthesis for esiRNA delivery to knock down the SOX-9 gene with high delivery efficiency. The self-assembled injectable system is further utilized for 3D cell culture, segregated co-culture of hMSC with human umbilical vein endothelial cells (HUVEC) as an angiogenesis model, and 3D bioprinting. Most interestingly, the coating of bioactive glass with the self-assembled biomatrix not only supports the proliferation and osteogenesis of hMSC in the 3D scaffold but also induces the amorphous bioactive glass (BG) scaffold surface to form new apatite crystals resembling bone-shaped plate structures. Thus, the self-assembled biomatrix system can be utilized in various dimensions, scales, and geometries for many different bioengineering applications.


Original languageEnglish
Article number2104758
Pages (from-to)1-14
Number of pages14
Issue number13
Early online date8 Feb 2022
Publication statusPublished - 8 Feb 2022

External IDs

WOS 000752320000001
Scopus 85124568086
Mendeley 8d3cefcc-f9cb-3d79-8926-d2e0d9b70cfc
unpaywall 10.1002/smll.202104758
ORCID /0000-0003-4191-715X/work/142240938
ORCID /0000-0003-2772-8504/work/142250996
ORCID /0000-0002-6669-4995/work/142251833



  • 3D printing, bioactive glass scaffolds, cell-bioengineering, extracellular matrix, injectable hydrogels, self-assembled matrix

Library keywords