Data from human studies imply that vanillin is an olfactory stimulant, whereas CO2 activates intranasal trigeminal nociceptors. We examined the effects of the olfactotoxin 3-methylindole (3-MI) on nasal mucosal potentials evoked by vanillin and CO2 in rats. A single i.p. administration of 300 mg/kg 3-MI altered both olfactory and trigeminal mucosal responses. Relative to amplitude values determined in non-3-MI-injected rats, the response to vanillin was reduced to 6%, 7%, and 43%, and the response to CO2, recorded in the same rats, decreased to 25%, 38%, and 51% at 4, 8 and 16 days post-3-MI, respectively. The results suggest that 3-MI affects both olfactory and trigeminal elements within the nasal mucosa. NeuroReport 11:2195-2197 (C) 2000 Lippincott Williams & Wilkins.