3D matrix-based cell cultures: Automated analysis of tumor cell survival and proliferation

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Three-dimensional ex vivo cell cultures mimic physiological in vivo growth conditions thereby significantly contributing to our understanding of tumor cell growth and survival, therapy resistance and identification of novel potent cancer targets. In the present study, we describe advanced three-dimensional cell culture methodology for investigating cellular survival and proliferation in human carcinoma cells after cancer therapy including molecular therapeutics. Single cells are embedded into laminin-rich extracellular matrix and can be treated with cytotoxic drugs, ionizing or UV radiation or any other substance of interest when consolidated and approximating in vivo morphology. Subsequently, cells are allowed to grow for automated determination of clonogenic survival (colony number) or proliferation (colony size). The entire protocol of 3D cell plating takes ~1 h working time and pursues for ~7 days before evaluation. This newly developed method broadens the spectrum of exploration of malignant tumors and other diseases and enables the obtainment of more reliable data on cancer treatment efficacy.

Details

Original languageEnglish
Pages (from-to)313-321
Number of pages9
JournalInternational Journal of Oncology
Volume48
Issue number1
Publication statusPublished - Jan 2016
Peer-reviewedYes

External IDs

Scopus 84953250723
PubMed 26549537
PubMedCentral PMC4734598
ORCID /0000-0001-5684-629X/work/147143553

Keywords

Sustainable Development Goals

Keywords

  • Carcinoma/drug therapy, Cell Culture Techniques/methods, Cell Line, Tumor, Cell Proliferation/drug effects, Cell Survival/drug effects, Extracellular Matrix/drug effects, Humans, Laminin/chemistry