1H-13C-29Si triple resonance and REDOR solid-state NMR - A tool to study interactions between biosilica and organic molecules in diatom cell walls

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Triple resonance solid-state NMR experiments using the spin combination 1H-13C-29Si are still rarely found in the literature. This is due to the low natural abundance of the two heteronuclei. Such experiments are, however, increasingly important to study hybrid materials such as biosilica and others. A suitable model substance, ideally labeled with both 13C and 29Si, is thus very useful to optimize the experiments before applying them to studies of more complex samples such as biosilica. Tetraphenoxysilane could be synthesized in an easy, two-step synthesis including double isotope labelling. Using tetraphenoxysilane, we established a 1H-13C-29Si double CP-based HETCOR experiment and applied it to diatom biosilica from the diatom species Thalassiosira pseudonana. Furthermore, we carried out 1H-13C{29Si} CP-REDOR experiments in order to estimate the distance between the organic matrix and the biosilica. Our experiments on diatom biosilica strongly indicate a close contact between polyamine-containing parts of the organic matrix and the silica. This corroborates the assumption that the organic matrix is essential for the control of the cell wall formation.

Details

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalSolid State Nuclear Magnetic Resonance
Volume66-67
Publication statusPublished - 5 Jan 2015
Peer-reviewedYes

External IDs

PubMed 25638422

Keywords

Keywords

  • C{Si} REDOR, Biomineralization, Diatom biosilica, Double CP, Tetraphenoxysilane, Triple resonance NMR