Viral infiltration of pancreatic islets in patients with COVID-19
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Metabolic diseases are associated with an increased risk of severe COVID-19 and conversely, new-onset hyperglycemia and complications of preexisting diabetes have been observed in COVID-19 patients. Here, we performed a comprehensive analysis of pancreatic autopsy tissue from COVID-19 patients using immunofluorescence, immunohistochemistry, RNA scope and electron microscopy and detected SARS-CoV-2 viral infiltration of beta-cells in all patients. Using SARS-CoV-2 pseudoviruses, we confirmed that isolated human islet cells are permissive to infection. In eleven COVID-19 patients, we examined the expression of ACE2, TMPRSS and other receptors and factors, such as DPP4, HMBG1 and NRP1, that might facilitate virus entry. Whereas 70% of the COVID-19 patients expressed ACE2 in the vasculature, only 30% displayed ACE2-expression in beta-cells. Even in the absence of manifest new-onset diabetes, necroptotic cell death, immune cell infiltration and SARS-CoV-2 viral infection of pancreatic beta-cells may contribute to varying degrees of metabolic dysregulation in patients with COVID-19.
Details
Originalsprache | Englisch |
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Aufsatznummer | 3534 |
Fachzeitschrift | Nature communications |
Jahrgang | 12 |
Ausgabenummer | 1 |
Publikationsstatus | Veröffentlicht - 1 Dez. 2021 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 34112801 |
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ORCID | /0000-0001-6287-9725/work/148607095 |
ORCID | /0000-0002-0320-4223/work/150884952 |