Transferrin receptor 2 deficiency promotes macrophage polarization and inflammatory arthritis

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

Rheumatoid arthritis is an inflammatory joint disease in which synovial iron deposition has been described. Transferrin receptor 2 (Tfr2) represents a critical regulator of systemic iron levels. Loss of Tfr2 function in humans and mice results in iron overload. As iron contributes to inflammatory processes, we investigated whether Tfr2-deletion affects the pathogenesis of inflammatory arthritis in an iron-dependent manner.

METHODS: Using a global and conditional genetic disruption of Tfr2, we assessed the relevance of Tfr2 in K/BxN serum-transfer arthritis (STA) and macrophage polarization.

RESULTS: Male Tfr2 -/- mice subjected to STA developed pronounced joint swelling, and bone erosion as compared to Tfr2 +/+ littermate-controls (P < 0.01). Furthermore, an increase of neutrophils and macrophages/monocytes was observed in the inflammatory infiltrate within the paws of Tfr2 -/- mice. To elucidate whether Tfr2 in myeloid cells has a direct role in the pathogenesis of arthritis or whether the effects were mediated via the systemic iron overload, we induced STA in Tfr2 fl/fl-LysMCre + mice, which showed normal iron-loading. Cre + female mice displayed increased disease development compared to Cre-controls. As macrophages regulate iron availability and innate immunity, we hypothesized that Tfr2-deficiency would polarize macrophages toward a pro-inflammatory state (M1) that contributes to arthritis progression. In response to IFN-γ stimulation, Tfr2 -/- macrophages showed increased expression of M1-like cytokines, IFN-γ-target genes, nitric-oxide production, and prolonged STAT1 activation compared to Tfr2 +/+ macrophages (P < 0.01), while pre-treatment with ruxolitinib abolished Tfr2-driven M1-like polarization.

CONCLUSION: Taken together, these findings suggest a protective role of Tfr2 in macrophages on the progression of arthritis via suppression of M1-like polarization.

Details

OriginalspracheEnglisch
Aufsatznummer102616
FachzeitschriftRedox biology
Jahrgang60
PublikationsstatusVeröffentlicht - Apr. 2023
Peer-Review-StatusJa

Externe IDs

PubMed 36746004
ORCID /0000-0002-8691-8423/work/142236070
ORCID /0000-0002-2061-8663/work/142246361
ORCID /0009-0001-6045-3349/work/150330117

Schlagworte

Forschungsprofillinien der TU Dresden

DFG-Fachsystematik nach Fachkollegium

Fächergruppen, Lehr- und Forschungsbereiche, Fachgebiete nach Destatis

Ziele für nachhaltige Entwicklung

Schlagwörter

  • Humans, Mice, Male, Female, Animals, Mice, Knockout, Iron/metabolism, Iron Overload/pathology, Macrophages/metabolism, Arthritis/metabolism, Receptors, Transferrin/genetics