Transcriptional Levels of Intercellular Junction Proteins in an Alveolar Epithelial Cell Line Exposed to Irradiation or Bleomycin

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

We performed a comparative study of the effects of X-ray irradiation and bleomycin on the mRNA levels of E-cadherin and tight junction proteins (claudin-3, claudin-4, claudin-18, ZO-2, and occludin) in an alveolar epithelial cell line L2. Irradiation decreased claudin-4 levels and increased occludin levels, while the levels of other mRNAs remained unchanged. Bleomycin increased the expression levels of all proteins examined except claudin-3. Irradiation and bleomycin have different effects on the expression level of intercellular junction proteins, indicating different reactions triggered in alveolar epithelial cells and a great prospects of further comparative studies.

Details

OriginalspracheEnglisch
Seiten (von - bis)442-446
Seitenumfang5
FachzeitschriftBulletin of Experimental Biology and Medicine
Jahrgang176
Ausgabenummer4
PublikationsstatusVeröffentlicht - Feb. 2024
Peer-Review-StatusJa

Externe IDs

PubMed 38488962
ORCID /0000-0003-3953-3253/work/158306270

Schlagworte

Schlagwörter

  • E-cadherin, bleomycin, cell line L2, irradiation, tight junctions, Claudin-3/metabolism, Occludin/genetics, Epithelial Cells, Bleomycin/pharmacology, Intercellular Junctions/metabolism, RNA, Messenger/genetics, Zonula Occludens-1 Protein/metabolism, Tight Junctions/metabolism, Alveolar Epithelial Cells/metabolism, Claudin-4/metabolism