Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation and whole embryo culture

Publikation: Beitrag in FachzeitschriftÜbersichtsartikel (Review)Beigetragen

Beitragende

Abstract

In mammals, embryonic development is more difficult to analyze than in non-mammalian species because this development occurs in utero. Interestingly, whole embryo culture allows the normal development of mouse post-implantation embryos for up to 2 days in vitro. One limitation of this technology has been the difficulty of performing loss-of-gene function studies in this system. RNA interference (RNAi), whereby double-stranded RNA molecules suppress the expression of complementary genes, has rapidly become a widely used tool for gene function analyses. We have combined the technologies of mouse whole embryo culture and RNAi to allow the molecular dissection of developmental processes. Here, we review the manipulation by topical injection followed by directional electroporation of endoribonuclease-prepared siRNA to demonstrate that this technology may be useful to knock down genes in a tissue- and region-specific manner in several organs of the developing mouse embryo.

Details

OriginalspracheEnglisch
Seiten (von - bis)92-102
Seitenumfang11
FachzeitschriftDifferentiation; research in biological diversity
Jahrgang72
Ausgabenummer2-3
PublikationsstatusVeröffentlicht - März 2004
Peer-Review-StatusNein

Externe IDs

Scopus 16644398519

Schlagworte

Schlagwörter

  • Animals, Electroporation, Embryo Culture Techniques/instrumentation, Embryo, Mammalian/anatomy & histology, Endoribonucleases/metabolism, Mice, Nervous System/cytology, RNA Interference, RNA, Small Interfering/genetics