Sulfated glycosaminoglycans exploit the conformational plasticity of bone morphogenetic protein-2 (BMP-2) and alter the interaction profile with its receptor

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

Sulfated glycosaminoglycans (GAGs) can direct cellular processes by interacting with proteins of the extracellular matrix (ECM). In this study we characterize the interaction profiles of chemically sulfated hyaluronan (HA) and chondroitin sulfate (CS) derivatives with bone morphogenetic protein-2 (BMP-2) and investigate their relevance for complex formation with the receptor BMPR-IA. These goals were addressed by surface plasmon resonance (SPR) and ELISA in combination with molecular modeling and dynamics simulation. We found not only the interaction of BMP-2 with GAGs to be dependent on the type and sulfation of GAGs but also BMP-2/GAG/BMPR-IA complex formation. The conformational plasticity of the BMP-2 N-termini plays a key role in the structural and thermodynamic characteristics of the BMP-2/GAG/BMPR-IA system. Hence we propose a model that provides direct insights into the importance of the structural and dynamical properties of the BMP-2/BMPR-IA system for its regulation by sulfated GAGs, in which structural asymmetry plays a key role.

Details

OriginalspracheEnglisch
Seiten (von - bis)3083-3092
Seitenumfang10
FachzeitschriftBiomacromolecules
Jahrgang15
Ausgabenummer8
PublikationsstatusVeröffentlicht - 11 Aug. 2014
Peer-Review-StatusJa

Externe IDs

Scopus 84905841944
ORCID /0000-0002-5611-9903/work/142244037

Schlagworte

Schlagwörter

  • Biocompatible Materials/chemistry, Biosensing Techniques, Bone Morphogenetic Protein 2/chemistry, Bone Morphogenetic Protein Receptors, Type I/chemistry, Chondroitin Sulfates/chemistry, Computational Biology, Extracellular Matrix/chemistry, Glycosaminoglycans/chemistry, Humans, Hyaluronic Acid/chemistry, Protein Conformation, Thermodynamics, Tissue Engineering

Bibliotheksschlagworte