Real-time monitoring of melting curves on DNA microarrays in plastic lab-on-foil system using silicon photomultiplier detectors
Publikation: Beitrag in Buch/Konferenzbericht/Sammelband/Gutachten › Beitrag in Konferenzband › Beigetragen › Begutachtung
Beitragende
Abstract
We report on DNA genotyping by melting curve analysis (MCA) in a plastic foil-microfluidic system using silicon photomultiplier (SiPM) detectors. Matching and single base mismatching DNA oligonucleotides were immobilized on thin-film foil microheaters and integrated in microfluidic channels using lamination foils. Two SiPMs, each with a sensitive area of 1.2×1.2 mm were assembled in an optical measurement setup to monitor the decaying Cy3 fluorescence intensity of the hybridized DNA spots upon melting. Successful detection of single nucleotide polymorphisms (SNP) is demonstrated.
Details
Originalsprache | Englisch |
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Titel | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
Herausgeber (Verlag) | Chemical and Biological Microsystems Society (CBMS) |
Seiten | 2294-2296 |
Seitenumfang | 3 |
ISBN (elektronisch) | 9780979806476 |
Publikationsstatus | Veröffentlicht - 2014 |
Peer-Review-Status | Ja |
Publikationsreihe
Reihe | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) |
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Konferenz
Titel | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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Dauer | 26 - 30 Oktober 2014 |
Stadt | San Antonio |
Land | USA/Vereinigte Staaten |
Externe IDs
ORCID | /0000-0002-0757-3325/work/139064930 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Lab-on-chip, Real-time melting curve analysis, Silicon photomultiplier, SNP detection