New sensitive method for the determination of hydrochlorothiazide in human serum by high-performance liquid chromatography with electrochemical detection
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
A HPLC method with a very sensitive electrochemical detection has been developed for determining the diuretic agent hydrochlorothiazide (HCT) in serum of volunteers to whom a single dose of the fixed combination of 12.5 mg HCT and 25 mg triamterene was administered. In the present method samples (0.2 ml serum, pH 7) were purified by extraction of HCT into 5 ml tert.-butyl-methyl ether. The separated organic phase was spiked with p-aminobenzoic acid (PABA) standard. The separation was performed on a reversed-phase C18 column using phosphate buffer-acetonitrile (90:10). A coulometric cell was used to measure HCT and an ultraviolet detector for PABA. The limit of quantitation for serum samples of only 200 μl was 5 ng/ml (i.e. 60 pg HCT/injection) with a good reproducibility (1-8%). Short retention times were found: 1.2 min for PABA and 5.8 min for HCT.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 293-296 |
Seitenumfang | 4 |
Fachzeitschrift | Journal of chromatography : including electrophoresis, mass spectrometry and other separation and detection methods : A |
Jahrgang | 729 |
Ausgabenummer | 1-2 |
Publikationsstatus | Veröffentlicht - 5 Apr. 1996 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 9004952 |
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ORCID | /0000-0003-1526-997X/work/142247278 |
Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Hydrochlorothiazide