Mutational status of KIT and PDGFRA and expression of PDGFRA are not associated with prognosis after curative resection of primary Gastrointestinal Stromal Tumors (GISTs)
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Beitragende
Abstract
Background: The aim of this study was to investigate if immunohistochemical expression and mutational status of KIT and PDGFRA in GISTs are associated with the clinical course and disease-free survival after curative resection of the primary tumor without adjuvant systemic therapy. Methods: Paraffin-embedded tumor sections of 95 GISTs were analyzed for KIT and PDGFRA expression by immunohistochemistry. PDGFRA expression was judged using a scoring system subdividing tumors in negative/weak and strong immunoreactivity groups. For mutation analysis, exons 9, 10, 11, 13, and 17 of KIT and exons 10, 12, 14, and 18 of PDGFRA were sequenced. Results: Of 95 R0-resected GISTs, 69% showed strong PDGFRA immunoreactivity. Gastric GISTs revealed a significantly higher rate of strong PDGFRA immunoreactivity (P = 0.01) and longer DFS (P = 0.015) than GISTs of the small intestine. KIT mutations were detected in 43 of 63 (68.3%) completely sequenced cases while PDGFRA mutations were identified in 6 cases (10%). In multivariate analysis, neither KIT/PDGFRA expression nor mutational status of KIT or PDGFRA were independent prognostic factors. Only mitotic rate predicted recurrence independently. Conclusion: Our data do not support the notion that expression of PDGFRA or mutations in KIT or PDGFRA are independent prognostic factors after curative resection of primary GIST.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 59-65 |
Seitenumfang | 7 |
Fachzeitschrift | Journal of surgical oncology |
Jahrgang | 104 |
Ausgabenummer | 1 |
Publikationsstatus | Veröffentlicht - 1 Juli 2011 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 21387320 |
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