Morphological changes in plasma-exposed poultry red mites (Dermanyssus gallinae) using high-resolution video camera and optical coherence tomography (OCT)

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Vanessa Rüster - , Friedrich-Loeffler-Institute (Autor:in)
  • Henrik Werner - , Hochschule für angewandte Wissenschaft und Kunst (HAWK) Hildesheim/Holzminden/Göttingen (Autor:in)
  • Georg Avramidis - , Hochschule für angewandte Wissenschaft und Kunst (HAWK) Hildesheim/Holzminden/Göttingen (Autor:in)
  • Stephan Wieneke - , Hochschule für angewandte Wissenschaft und Kunst (HAWK) Hildesheim/Holzminden/Göttingen (Autor:in)
  • Christina Strube - , Stiftung Tierärztliche Hochschule Hannover (TiHo) (Autor:in)
  • Christian Schnabel - , Klinik und Poliklinik für Anaesthesiologie und Intensivtherapie (Gemeinsame:r Letztautor:in)
  • Thomas Bartels - , Friedrich-Loeffler-Institute (Gemeinsame:r Letztautor:in)

Abstract

Dermanyssus gallinae, the poultry red mite (PRM), is a hematophagous temporary ectoparasite that causes serious economic losses and animal health impairment on laying hen farms worldwide. Control is limited by the parasite's hidden lifestyle, restrictions on the use of chemical acaricides and the development of resistance against certain drug classes. As a result, research was conducted to explore alternative control methods. In recent years, atmospheric pressure plasma has been increasingly reported as an alternative to chemical acaricides for pest control. This physical method has also shown promising against PRM under laboratory conditions. However, the detailed mechanisms of action have not yet been elucidated. In the present study, the effects of cold atmospheric pressure plasma on PRM were investigated using digital videography and optical coherence tomography (OCT), an imaging technique that visualizes the topography of surfaces and internal structures. Digital videography showed that a redistribution of the contents of the intestinal tract and excretory organs (Malpighian tubules) occurred immediately after plasma exposure. The body fluids reached the distal leg segments of PRM and parts of the haemocoel showed whiter and denser clumps, indicating a coagulation of the haemocoel components. OCT showed a loss of the boundaries of the hollow organs in transverse and sagittal sectional images as well as in the three-dimensional image reconstruction. In addition, a dorso-ventral shrinkage of the idiosoma was observed in plasma-exposed mites, which had shrunk to 44.0% of its original height six minutes after plasma exposure.

Details

OriginalspracheEnglisch
Seiten (von - bis)339-352
Seitenumfang14
FachzeitschriftExperimental & applied acarology
Jahrgang93
Ausgabenummer2
Frühes Online-Datum27 Juni 2024
PublikationsstatusVeröffentlicht - Aug. 2024
Peer-Review-StatusJa

Externe IDs

ORCID /0000-0002-7267-7016/work/163294974
Scopus 85197871351

Schlagworte