Modulated fibronectin anchorage at polymer substrates controls angiogenesis

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Max Bergmann Zentrum für Biomaterialien Dresden (MBZ)

Abstract

A set of maleic anhydride copolymer thin films exhibiting well-defined differences in hydrophobicity and reactivity was compared with respect to the capability of supporting angiogenesis of human endothelial cells grown in contact. The physicochemical surface characteristics of the polymer substrates were found to modulate the anchorage of immobilized fibronectin. This was demonstrated to determine whether endothelial cells grow as a monolayer or form capillary networks. Enhanced reorganization of predeposited fibronectin into cell-matrix adhesions and slightly elevated levels of membrane-type matrix metalloproteinase 14 (MMP-14) occurred with weakly bound fibronectin layers where angiogenesis was most obvious. The key role of fibronectin-substrate binding for angiogenesis-under otherwise constant conditions-was further confirmed by the absence of variations in the expression of angiogenesis-related integrins (alpha(v)beta(3)) and in the secretion of the metalloproteinase MMP-2. Altogether, the results of this study point at the relevance of physicochemical surface characteristics of polymer materials for the stimulation of angiogenesis.

Details

OriginalspracheEnglisch
Seiten (von - bis)841-8
Seitenumfang8
FachzeitschriftTissue engineering
Jahrgang10
Ausgabenummer5-6
PublikationsstatusVeröffentlicht - 2004
Peer-Review-StatusJa

Externe IDs

Scopus 3042735324
ORCID /0000-0003-0189-3448/work/173985706

Schlagworte

Schlagwörter

  • Cell Adhesion/physiology, Cell Culture Techniques/methods, Cells, Cultured, Coated Materials, Biocompatible/chemistry, Endothelial Cells/cytology, Extracellular Matrix/physiology, Extracellular Matrix Proteins/metabolism, Fibronectins/chemistry, Humans, Maleic Anhydrides/chemistry, Materials Testing, Neovascularization, Physiologic/physiology, Polymers/chemistry, Surface Properties, Tissue Engineering/methods