Gradient multi-materials combining 316L steel and Inconel 625 superalloy are needed for components operating at variable temperature and load conditions. This study investigates for the first time the laser powder bed fusion manufacturing of 316L/Inconel 625 gradient multi-materials by step-like varying the composition. Tomographic and microscopy analysis revealed low porosity and few microcracks. Its presence does not affect mechanical properties. Mixing 316L with Inconel 625 favors the microstructure with small equiaxed grains and precipitates of the Laves phase and carbides along cell and grain boundaries. The phase analysis of precipitates by electron diffraction is in line with Thermo-Calc calculations and shows that higher Nb and Mo content promotes precipitation of M₂₃C₆ carbides instead of M₆C. The increase in hardness across the gradient is mainly attributed to solid solution strengthening by Nb and Mo. The transverse arrangement of the gradient sample in tensile test results in higher strength than in a longitudinal orientation. This study shows that the designed process conditions and gradient scheme reduce the risk for abrupt element segregation, leading to brittleness, typical for directed energy deposition. The interplay between manufacturing, chemical composition, microstructure and mechanical properties of gradient multi-material is determined.