Measurements of one-way delay in 5G networks are essential for evaluating system performance in various practical scenarios. This paper presents a comprehensive study comparing three different packet generators on real-world deployments and their associated challenges. We explore different topolo-gies, types of terminals, and network congestion conditions, discussing the suitability and limitations of different packet generators. Moreover, we propose practical considerations to overcome limitations in capturing dynamic and heterogeneous 5G network environments and provide insights for system designers in precisely measuring and characterizing one-way delay in 5G networks for real-world scenarios.