The present paper deals with the experimental investigation of interlocking effect of crushed stone ballast material, assessing it as the relationship with the residual and dynamic stresses under the ballast layer during laboratory dynamic tests with the consideration of different boundary conditions. The laboratory experiments were executed with a scaled model of ballast under the sleeper. The measured pressure at the bottom surface of the ballast has two parts: dynamic and residual. The dynamic part depends on the external loading; the residual part remains after unloading. The measured residual stress was observed up to 3 times higher than the stress due to cyclic external loading. The relationship of the residual stress and interlocking effect to ballast particles angularity is analyzed. A simple interpretation of the distribution of residual stress is proposed, that depends on the measured cyclic stress and the elasticity of bounding walls. The study of interlocking effect of ballast could be potentially useful for many practical problems of railway track design as well as for the track maintenance issues.