Water, energy and food are the three foremost vital resources that need to be protected for human existence and sustainable development. The study develops an indicator-based framework for integrated assessment of the water–energy–food nexus at the river basin scale. The framework is designed to have three successive levels for the assessment. The proposed three-tiered assessment framework can aid concerned resources management authorities to evaluate the water–energy–food nexus and also to identify the action required to enhance resource productivities. The suitability of the framework is demonstrated through its application to the Ping River basin in Thailand.