Heavy Metal Stabilization of DNA Origami Nanostructures
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
DNA origami is a powerful tool to fold 3-dimensional DNA structures with nanometer precision. Its usage, however, is limited as high ionic strength, temperatures below ∼60 °C, and pH values between 5 and 10 are required to ensure the structural integrity of DNA origami nanostructures. Here, we demonstrate a simple and effective method to stabilize DNA origami nanostructures against harsh buffer conditions using [PdCl4]2-. It provided the stabilization of different DNA origami nanostructures against mechanical compression, temperatures up to 100 °C, double-distilled water, and pH values between 4 and 12. Additionally, DNA origami superstructures and bound cargos are stabilized with yields of up to 98%. To demonstrate the general applicability of our approach, we employed our protocol with a Pd metallization procedure at elevated temperatures. In the future, we think that our method opens up new possibilities for applications of DNA origami nanostructures beyond their usual reaction conditions.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 2429-2436 |
Seitenumfang | 8 |
Fachzeitschrift | Nano letters |
Jahrgang | 24 |
Ausgabenummer | 8 |
Publikationsstatus | Veröffentlicht - 28 Feb. 2024 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 38363878 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- DNA metallization, DNA nanostructures, DNA origami, DNA−metal interaction, seeded growth