Fast and label-free intraoperative discrimination of malignant pancreatic tissue by attenuated total reflection infrared spectroscopy

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

Significance: Pancreatic surgery is a highly demanding and routinely applied procedure for the treatment of several pancreatic lesions. The outcome of patients with malignant entities crucially depends on the margin resection status of the tumor. Frozen section analysis for intraoperative evaluation of tissue is still time consuming and laborious. Aim: We describe the application of fiber-based attenuated total reflection infrared (ATR IR) spectroscopy for label-free discrimination of normal pancreatic, tumorous, and pancreatitis tissue. A pilot study for the intraoperative application was performed. Approach: The method was applied for unprocessed freshly resected tissue samples of 58 patients, and a classification model for differentiating between the distinct tissue classes was established. Results: The developed three-class classification model for tissue spectra allows for the delineation of tumors from normal and pancreatitis tissues using a probability score for class assignment. Subsequently, the method was translated into intraoperative application. Fiber optic ATR IR spectra were obtained from freshly resected pancreatic tissue directly in the operating room. Conclusion: Our study shows the possibility of applying fiber-based ATR IR spectroscopy in combination with a supervised classification model for rapid pancreatic tissue identification with a high potential for transfer into intraoperative surgical diagnostics.

Details

OriginalspracheEnglisch
Aufsatznummer045004
Seitenumfang14
FachzeitschriftJournal of biomedical optics
Jahrgang28
Ausgabenummer4
PublikationsstatusVeröffentlicht - 1 Apr. 2023
Peer-Review-StatusJa

Externe IDs

PubMed 37122477
WOS 000985185200006
ORCID /0000-0002-8160-3000/work/142248356
ORCID /0000-0003-0554-2178/work/142249920
ORCID /0000-0002-7625-343X/work/150881420

Schlagworte

Schlagwörter

  • attenuated total reflection infrared, fiber-based spectroscopy, infrared spectroscopy, pancreas, pancreatic tumor, Pancreatic tumor, Infrared spectroscopy, Attenuated total reflection infrared, Fiber-based spectroscopy, Pancreas, Pancreatitis/diagnostic imaging, Humans, Pancreatic Neoplasms/diagnostic imaging, Spectroscopy, Fourier Transform Infrared/methods, Pilot Projects, Spectrophotometry, Infrared