Distinguishing autocrine and paracrine signals in hematopoietic stem cell culture using a biofunctional microcavity platform

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Eike Müller - , Leibniz Institute of Polymer Research Dresden (Autor:in)
  • Weijia Wang - , University of Toronto (Autor:in)
  • Wenlian Qiao - , University of Toronto (Autor:in)
  • Martin Bornhäuser - , Medizinische Klinik und Poliklinik I, Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)
  • Peter W. Zandstra - , University of Toronto (Autor:in)
  • Carsten Werner - , Professur für Biofunktionale Polymermaterialien (gB/IPF), Leibniz Institute of Polymer Research Dresden (Autor:in)
  • Tilo Pompe - , Leibniz Institute of Polymer Research Dresden, Universität Leipzig (Autor:in)

Abstract

Homeostasis of hematopoietic stem cells (HSC) in the mammalian bone marrow stem cell niche is regulated by signals of the local microenvironment. Besides juxtacrine, endocrine and metabolic cues, paracrine and autocrine signals are involved in controlling quiescence, proliferation and differentiation of HSC with strong implications on expansion and differentiation ex vivo as well as in vivo transplantation. Towards this aim, a cell culture analysis on a polymer microcavity carrier platform was combined with a partial least square analysis of a mechanistic model of cell proliferation. We could demonstrate the discrimination of specific autocrine and paracrine signals from soluble factors as stimulating and inhibitory effectors in hematopoietic stem and progenitor cell culture. From that we hypothesize autocrine signals to be predominantly involved in maintaining the quiescent state of HSC in single-cell niches and advocate our analysis platform as an unprecedented option for untangling convoluted signaling mechanisms in complex cell systems being it of juxtacrine, paracrine or autocrine origin.

Details

OriginalspracheEnglisch
Aufsatznummer31951
FachzeitschriftScientific reports
Jahrgang6
PublikationsstatusVeröffentlicht - 18 Aug. 2016
Peer-Review-StatusJa

Externe IDs

PubMed 27535453
ORCID /0000-0003-0189-3448/work/161890431

Schlagworte

ASJC Scopus Sachgebiete