Validation of the antimicrobial performance of contact-killing polymer surfaces through the experimental determination of bacterial adhesion or viability, is essential for their targeted development and application. However, there is not yet a consensus on the single most appropriate evaluation method or procedure. Combining and benchmarking previously reported assays could reduce the significant variation and misinterpretation of efficacy data obtained from different methods. In this work, we systematically investigated the response of bacterial cells to antiadhesive and antiseptic polymer coatings by combining (i) bulk solution-based, (ii) thin-film spacer-based, and (iii) direct-contact assays. In addition, we evaluated the studied assays using a five-point scoring framework that highlights key areas for improvement. Our data suggest that combined microscopy assays provide a more comprehensive representation of antimicrobial performance, thereby helping to identify effective types of antibacterial polymer coatings.