Binding of chondroitin 4-sulfate to cathepsin S regulates its enzymatic activity

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Juliette Sage - , INSERM - Institut national de la santé et de la recherche médicale (Autor:in)
  • Florian Mallèvre - (Autor:in)
  • Fabien Barbarin-Costes - (Autor:in)
  • Sergey A Samsonov - , Strukturelle Bioinformatik (FoG), Biotechnologisches Zentrum (BIOTEC) (Autor:in)
  • Jan-Philip Gehrcke - , Biotechnologisches Zentrum (BIOTEC) (Autor:in)
  • Maria Teresa Pisabarro - , Strukturelle Bioinformatik (FoG), Biotechnologisches Zentrum (BIOTEC) (Autor:in)
  • Eric Perrier - (Autor:in)
  • Sylvianne Schnebert - (Autor:in)
  • André Roget - (Autor:in)
  • Thierry Livache - (Autor:in)
  • Carine Nizard - (Autor:in)
  • Gilles Lalmanach - (Autor:in)
  • Fabien Lecaille - , INSERM - Institut national de la santé et de la recherche médicale (Autor:in)

Abstract

Human cysteine cathepsin S (catS) participates in distinct physiological and pathophysiological cellular processes and is considered as a valuable therapeutic target in autoimmune diseases, cancer, atherosclerosis, and asthma. We evaluated the capacity of negatively charged glycosaminoglycans (heparin, heparan sulfate, chondroitin 4/6-sulfates, dermatan sulfate, and hyaluronic acid) to modulate the activity of catS. Chondroitin 4-sulfate (C4-S) impaired the collagenolytic activity (type IV collagen) and inhibited the peptidase activity (Z-Phe-Arg-AMC) of catS at pH 5.5, obeying a mixed-type mechanism (estimated Ki = 16.5 ± 6 μM). Addition of NaCl restored catS activity, supporting the idea that electrostatic interactions are primarly involved. Furthermore, C4-S delayed in a dose-dependent manner the maturation of procatS at pH 4.0 by interfering with the intermolecular processing pathway. Binding of C4-S to catS was demonstrated by gel-filtration chromatography, and its affinity was measured by surface plasmon resonance (equilibrium dissociation constant Kd = 210 ± 40 nM). Moreover, C4-S induced subtle conformational changes in mature catS as observed by intrinsic fluorescence spectroscopy analysis. Molecular docking predicted three specific binding sites on catS for C4-S that are different from those found in the crystal structure of the cathepsin K-C4-S complex. Overall, these results describe a novel glycosaminoglycan-mediated mechanism of catS inhibition and suggest that C4-S may modulate the collagenase activity of catS in vivo.

Details

OriginalspracheEnglisch
Seiten (von - bis)6487-6498
Seitenumfang12
Fachzeitschrift Biochemistry : a weekly publication of the American Chemical Society
Jahrgang52
Ausgabenummer37
PublikationsstatusVeröffentlicht - 17 Sept. 2013
Peer-Review-StatusJa

Externe IDs

Scopus 84884275724

Schlagworte

Ziele für nachhaltige Entwicklung

Schlagwörter

  • Binding Sites, Cathepsins/antagonists & inhibitors, Chondroitin Sulfates/metabolism, Chromatography, Gel, Collagen Type IV/metabolism, Coumarins/metabolism, Dipeptides/metabolism, Humans, Kinetics, Molecular Docking Simulation, Molecular Dynamics Simulation, Surface Plasmon Resonance